ANALYSIS OF MICROSATELLITE LOCI POLIMORPHISM OF TOMATO GENOTYPES WITH DIFFERENT RESISTANCE LEVEL TO FUSARIUM WILT OF TOMATO

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04.02.2015

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Abstract

As result of conducted researches 5 pair of direct and reverse SSR primers (SSR14, SSR105, SSR111, SSR266, SSR295) were evaluated to detect both intraspecific and intraspecific polymorphism of tomato. An analysis of 9 forms tomato with different resistance to Fusarium of tomato were conducted using 5 microsatellite loci. As a results of molecular investigation 19 allelic variants (from 2 to 5 per locus) were determinated that equal 3.8 alleles per microsatellite locus. Among researched microsatellite loci SSR14 and SSR105 had most polymorphic potential, because consist 5 alleles per locus. Genetic passports of two genotypes-standards (La4026 and F₇Orko lines (L. esculentum (Mill.))) of resistance to Fusarium wilt of tomato were created. Genetic identification of four semicultural form of tomato (L. esculentum var. pimpinellifolium (Mill.) Brezh., L. esculentum var. cerasiforme (A. Gray) Brezh., L. cheesmanii var. typicus Riley, L. esculentum var. racemigerum (Lange) Brezh.), which are donors of abiotic and biotic resistance and perspective forms for introgression of embryonic plasma into gene pool cultural form of tomato, were conducted. 

Keywords

tomato, polymorphism, genome, microsatellite loci, SSR primers, genetic passports.