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SOURCES OF HIGH AMYLOPECTIN CONTENT IN STARCH IN SPRING BARLEY BREEDING

SOURCES OF HIGH AMYLOPECTIN CONTENT IN STARCH IN SPRING BARLEY BREEDING

Institution

V. Ya. Yuryev Plant Production Institute of NAAN

Section

SOURCES AND DONORS

Abstract

Goal. The goal of this research was to create lines with high amylopectin content in starch via using waxy barley forms in hybridization and to evaluate them in a cultivar trial.

Materials and Methods. The starting material for hybridization was waxy barley accessions UA 039699 and UA 039701 as well as cultivars ‘Dzherelo’, ‘Etiket’, ‘Aspepkt’, ‘Philadelphia’, and accession IR 6576. Waxy barley lines were grown and evaluated in a competitive cultivar trial by the state cultivar trial procedure in 2014-2015. A drill SSFK 7 equipped with a batch dropper was used for sowing. The plot area - 10.5 m2. The seeding rate - 4.5 million seeds / ha. The yield capacity was compared with the standard ‘Vzirets’. Phenological observations were carried out, with recording the seedling, earing and grain ripeness phases. Lodging resistance was assessed using 9-point scale. LSD0.5 was determined by analysis of variance according to the BA Dospekhov method. 

Results and Discussion. As a result of use of spring barley forms with various amylopectin content in starch (collection waxy accessions UA 039699 и UA 039701 with high amylopectin content and accessions with standard starch) in breeding, the effectiveness of creation of high-yielding lines with starch, which almost completely consist of amylopectin, was shown. Variety composition of lines with amylopectin starch was expanded: lines belonging to nutans, medicum and rikotense varieties were obtained, while the original waxy accessions belonged to medicum variety. In 2014-2015, the cultivar trial distinguished the best eight lines, of which seven lines (12-945, 12-954, 12-965, 12-1014, 12-333, 12-473, 12-476) had high yields (107 %, 105 %, 105 %, 116 %, 110 %, 107 %, 115 % related to the standard, respectively) and lodging resistance (8.5-9.0 points) with an equal or shorter duration of the vegetation period. Lines 12-945 (medicum; 82 days vs. 87 days in the standard), 12-954 (medicum; 82 days vs. 87 days in the standard), 12-965 (nutans; 82 days), 12 -333 (nutans; 82 days) were more early-ripening than the standard ‘Vzirets’. Lines 12-1014 (medicum; 84 days), 12-476 (rikotense; 84 days), 12-408 (nutans; 85 days), 12-473 (rikotense; 85 days) had the vegetation period duration on the standard level. The distinguished waxy lines are sources of high amylopectin content in starch and of valuable economic features.

Conclusions. The effectiveness of breeding of high-yielding waxy barley lines was shown. Waxy barley lines with yielding capacity increased by 5–16 % and high lodging resistance (8.5-9.0 points) were identified.  

Keywords

spring, barley, line, waxy, amylopectin, variety, yield capacity, lodging resistance, weight, vegetation